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Wuyuan Lu
Adjunct Associate Professor
B.S. Nanjing University, China 1984; Ph.D. Purdue University, West Lafayette 1994; Post-Doctoral The Scripps Research Institute, La Jolla 1996
Wu, Z., Powell, R. and Lu, W. (2003). Productive folding of human neutrophil alpha-defensins in vitro without the pro-peptide. J. Am. Chem. Soc. 125, 2402-2403.
Wu, Z., Hoover, D.M., Yang, D., Boulegue, C., Santamaria, F., Oppenheim, J., Lubkowski, J. and Lu, W. (2003). Engineering disulfide bridges to dissect antimicrobial and chemotactic activities of human beta-defensin 3. Proc. Natl. Acad. Sci. USA 100, 8880-8885.
Wu, Z., Prahl, A., Powell, R., Ericksen, B., Lubkowski, J. and Lu, W. (2003). From pro defensins to defensins: synthesis and characterization of human neutrophil pro alpha-defensin-1 and its mature domain. J. Pept. Res. 62, 53-62.
Pál, G., Santamaria, F., Kossiakoff, A. and Lu, W. (2003). The first semi-synthetic serine protease made by native chemical ligation. Protein Expres. Purif. 29, 185-192.
Qasim, M.A., Lu, W., Lu, S., Ranjbar, M., Yi, Z-P, Chiang, Y-W, Ryan, K., Anderson, S., Zhang, W., Qasim, S. and Laskowski, M. Jr. (2003). Testing of the additivity based protein sequence to reactivity algorithm. Biochemistry 42, 6460-6466.
Santamaria, F., Wu, Z., Boulegue, C., Pal, G. and Lu, W. (2003). Reexamination of the recognition preference of the specificity pocket of the Abl SH3 domain by targeted synthetic peptide libraries. J. Mol. Recognit. 16, 131-138.
Hoover, D.M., Wu, Z., Lu, W. and Lubkowski, J. (2003). Functional characterization of the antimicrobial activities of human beta-defensin-3 using peptide analogs. Antimicrob. Agents Chemother. 47, 2804-2809.
Wu, Z., Alexandratos, J., Ericksen, B., Lubkowski, J., Gallo, R.C. and Lu, W. (2004). Total chemical synthesis of N-myristoylated HIV-1 matrix protein p17: Structural and mechanistic implications of p17 myristoylation. Proc. Natl. Acad. Sci. USA 101, 11587-11592.
Cai, Z., Xu, C., Xu, Y., Lu, W., Chi, C-W., Shi, Y. and Wu, J. (2004). Solution structure of BmBKTx1, a new BKCa1 channel blocker from the Chinese scorpion Buthus martensi Karsch. Biochemsitry 43, 3764-3771.
Szyka, A., Lu, W., Xu, C. and Lubkowski, J. (2004). Structure of the scorpion toxin BmBKTx1 solved from single wavelength anomalous scattering of sulfur. J. Struct. Biol. 145, 289-294.
Xu, C.Q., Brone, B., Wicher, D., Bozkurt, O., Lu, W., Huys, I., Han, Y.H., Tytgat, J., Van Kerkhove, E. and Chi, C-W. (2004). BmBKTx1, a novel Ca2+-activated K+ channel blocker purified from the Asian scorpion Buthus Martensi Karsch. J. Biol. Chem. 279, 34562-34569.
Wu, Z., Ericksen, B., Tucker, K., Lubkowski, J. and Lu, W. (2004). Synthesis and characterization of human alpha-defensins 4-6. J. Pept. Res. 64, 118-125.
Ericksen, B., Wu, Z., Lu, W. and Lehrer, R.I. (2005). Bactericidal activities and specificities of all six synthetic human alpha-defensins. Antimicrob. Agents Chemother. 49, 269-275.
Wu, Z., Cocchi, F., Gentles, D., Ericksen, B., Lubkowski, J., DeVico, A., Lehrer, R.I. and Lu, W. (2005). Human neutrophil alpha-defensin 4 inhibits HIV-1 infection in vitro. FEBS Lett. 579, 162-166.
Lim, J.K., Burns, J.M., Lu, W. and DeVico, A.L. (2005). Characterization of post-translational RANTES/CCL5 processing by human peripheral blood mononuclear cells: elucidation of a novel 4-68 isoform. J. Leukoc. Biol. 78, 442-52.
Xie, C., Zeng, P., Ericksen, B., Wu, Z., Lu, W-Y. and Lu, W. (2005). Effects of the terminal charges in human neutrophil alpha-defensin 2 on its bactericidal and membrane activity. Peptides 26, 2377-83.
Xie, C., Prahl, A., Ericksen, B., Wu, Z., Zeng, P., Li, X., Lu, W-Y., Lubkowski, J. and Lu, W. (2005). Reconstruction of the conserved beta-bulge in mammalian defensins using D-amino acids. J. Biol. Chem. 280, 32921-32929.
John, M., Keller, M.J., Fam, E.H., Cheshenko, N., Hogarty, K., Kasowitz, A., Wallenstein, S., Carlucci, M.J., Tuyama, A.C., Lu, W., Lehrer, R.I., Klotman, M.E. and Herold, B.C. (2005). Cervicovaginal secretions contribute to innate resistance to HSV infection. J. Infect. Dis. 192, 1731-40.
Economopoulou, M., Bdeir, K., Cines, D.B., Fogt, F., Bdeir, Y., Lubkowski, J., Lu, W., Preissner, K.T., Hammes, H-P. and Chavakis, T. (2005). Inhibition of pathological retinal neovascularization by alpha-defensins. Blood 106, 3831-8.
Sun, L., Finnegan, C., Kish-Catalone, T., Blumenthal, R., Garzino-Demo, P., La Terra Maggiore, G.M., Berrone, S., Kleinman, C., Wu, Z., Abdelwahab, S., Lu, W. and Garzino-Demo, A. (2005). Human beta-defensins suppress HIV infection: potential role in mucosal protection. J. Virol. 79, 14318-29.
Li, X., de Leeuw, E. and Lu, W. (2005). Total chemical synthesis of human psoriasin by native chemical ligation. Biochemistry 44, 14688-94.
Wu, Z., Li, X., de Leeuw, E., Ericksen, B. and Lu, W. (2005). Why is the Arg5-Glu13 salt bridge conserved in mammalian alpha-defensins? J. Biol. Chem. 280, 43039-43047.
Buck, C.B., Day, P.M., Thompson, C.D., Lubkowski, J., Lu, W., Lowy, D.R. and Schiller J.T. (2006). Human alpha-defensins block papillomavirus infection. Proc. Natl. Acad. Sci. USA 103, 1516-21.
Kish-Catalone, T.M., Lu, W.,Gallo, R.C. and DeVico, A.L. (2006). Preclinical evaluation of synthetic -2 RANTES as a candidate vaginal microbicide to target CCR5. Antimicrob. Agents Chemother. 50, 1497-509.
Wang, W., Mulakala, C., Ward, S.C., Jung, G., Luong, H., Pham, D., Waring, A.J., Kaznessis, Y., Lu, W., Bradley, K.A. and Lehrer, R.I. (2006). Retrocyclins kill bacilli and germinating spores of Bacillus anthracis and inactivate anthrax lethal toxin. J. Biol. Chem. 281(43):32755-64.
Lim, J.K., Lu, W., Hartley, O. and DeVico, A.L. (2006). N-terminal proteolytic processing by cathepsin G converts RANTES/CCL5 and related analogs into a truncated 4-68 variant. J. Leukoc. Biol. 80(6):1395-404.
Yi, ZP., Vitek, O., Qasim, M.A., Lu, S.M., Lu, W., Ranjbar, M., Li, J., Laskowski, M.C., Bailey-Kellogg, C. and Laskowski, M., Jr. (2006). Functional evolution with a protein superfamily. PROTEINS: Structure, Function, and Bioinformatics 63, 697-708.
De Leeuw, E., Li, X. and Lu, W. (2006). Binding characteristics of the Lactobacillus brevis ATCC 8287 surface layer to extra cellular matrix proteins. FEMS Microbiol. Lett. 260, 210-215.
Szyk, A., Wu, Z., Tucker, K., Yang, D., Lu, W. and Lubkowski, J. (2006). Crystal structures of human alpha-defensins HNP4, HD5, and HD6. Protein Sci. 15(12):2749-60.
Hazrati, E., Galen, B., Lu, W., Wang, W., Ouyang, Y., Keller, M.J., Lehrer, R.I. and Herold, B.C. (2006). Human alpha- and beta-defensins block multiple steps in herpes simplex virus infection. J. Immunol. 177, 8658-66.
Li, X., Zou, G., Yuan, W. and Lu, W. (2007). Defining the native disulfide topology in the somatomedin B domain of human vitronectin. J. Biol. Chem. 282(8):5318-26.
De Leeuw, E., Burks, S.R., Li, X., Kao, J.P.Y. and Lu, W. (2007). Structure-dependent functional properties of human defensin 5. FEBS Lett. 581(3):515-520.
Wu, Z., Li, X., Ericksen, B., De Leeuw, E., Zou, G., Zeng, P., Xie, C., Li, C., Lubkowski, J., Lu, W-Y. and Lu, W. (2007). Impact of pro segments on the folding and function of human neutrophil alpha-defensins. J. Mol. Biol. 368(2):537-549.
Zou, G., de Leeuw, E., Li, C., Pazgier, M., Li, C., Zeng, P., Lu, W.-Y., Lubkowski, J. and Lu, W. (2007). Toward understanding the cationicity of defensins: Arg and Lys versus their noncoded analogs. J. Biol. Chem. 282(27):19653-65.
Shi, J., Aono, S., Lu, W., Ouellette, A.J., Hu, X., Ji, Y., Wilson, C., Parks, W., Wang, L., Lenz, S., Dykstra, C., Morrison, E.E. and Elson, C.O. (2007). A novel role for defensins in intestinal homeostasis: regulation of IL-1beta secretion. J. Immunol. 179(2):1245-53.
Saad, J.S., Kim, A., Ghanam, R.H., Dalton, A.K., Vogt, V.M., Wu, Z., Lu, W. and Summers, M.F. (2007). Mutations that mimic phosphorylation of the HIV-1 matrix protein do not perturb the myristyl switch. Protein Sci. (in press).
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