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CONTACT INFORMATION
Office: IHV/UMD Medical School, 725 Lombard St.
Phone: 410-706-4982
Professional Interests
Publications
Honors/Awards
Funding
Courses Taught
Research Group
Lai-Xi Wang
Adjunct Associate Professor
Ph.D. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 1991; Post-Doctoral Johns Hopkins University 1994; Post-Doctoral Massachusetts Institute of Technology (MIT) 1997
SELECTED PUBLICATIONS

1. Ochiai, H., Huang, W., Wei, Y., Wang, L. X., “Expeditious chemoenzymatic synthesis of homogeneous N-glycoproteins carrying defined oligosaccharide ligands”. Journal of American Chemical Society, 2008, 130, 13790-13803 (2008).

 

2. Huang, W., Ochiai, H., Zhang, X., Wang, L. X., “Introducing N-glycans into natural products through a chemoenzymatic approach”, Carbohydrate Research, in press (2008).

 

3. Wei, Y., Li, C., Huang, W., Li, B., Strome, S. E., Wang, L. X., “Glyco-engineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation”, Biochemistry, 47, 10294-10304 (2008).

 

4. Lin, W., Voskens, C.J., Zhang, X., Schindler, D.G., Wood, A., Burch, E., Wei, Y., Chen, L., Tian, G., Tamada, K., Wang, L.X., Schulze, D.H., Mann, D., Strome, S.E., Fc dependent expression of CD137 on human NK cells: insights into 'agonistic' effects of anti-CD137 monoclonal antibodies”, Blood, 112, 699-707 (2008).

 

5. Li, C., Huang, W., Wang, L. X., “Chemoenzymatic synthesis of N-linked neoglycoproteins through a chitinase-catalyzed transglycosylation”, Bioorganic & Medicinal Chemistry, 16, 8366-8372 (2008).

 

6. Li, B., Takegawa, K., Suzuki, T., Yamamoto, K., Wang, L. X., “Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-N-acetylglucosaminidases”, Bioorganic & Medicinal Chemistry, 16, 4670-4675 (2008).

 

7. Wang, L. X., “Chemoenzymatic synthesis of glycopeptides and glycoproteins through endoglycosidase-catalyzed transglycosylation”, Carbohydrate Research, 343, 1509-22 (2008).

 

8. Umekawa, M., Huang, W., Li, B., Fujita, K., Ashida, H., Wang, L. X.,* Yamamoto, K.,* “Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities”, Journal of Biological Chemistry, 283, 4469-4479 (2008).

 

9. Li, X., Wang, L. X., Wang, X., Roseman, S., “The chitin catabolic cascade in the marine bacterium Vibrio Cholerae: Characterization of a unique chitin oligosaccharide deacetylase”. Glycobiology, 17, 1377-1387 (2007).

 

10. Li, H. G., Guan, Y., Szczepanska, A., Moreno-Vargas, A. J., Carmona, A. T., Robina, I., Lewis, G. K., Wang, L. X., 'Synthesis and anti-HIV activity of trivalent CD4-mimetic miniproteins', Bioorganic & Medicinal Chemistry, 15, 4220-4228 (2007).

 

11. Wang, J., Li, H., Zou, G., Wang, L. X., 'Novel template-assembled oligosaccharide clusters as epitope mimics for HIV-neutralizing antibody 2G12. Design, synthesis, and antibody binding study', Organic & Biomolecular Chemistry, 5, 1529-1540 (2007).

 

12. Wang, L. X., 'Toward oligosaccharide- and glycopeptide-based HIV vaccines' Current Opinion in Drug Discovery and Development, 9, 194-206 (2006).

 

13. Li, B., Song, H., Hauser, S., Wang, L. X. 'A highly efficient chemoenzymatic approach toward glycoprotein synthesis', Organic Letters, 8, 3081-3084 (2006).

 

14. Zeng, Y., Wang, J., Li, B., Hauser, S., Li, H., Wang, L. X.  'Glycopeptide synthesis through endoglycosidase-catalyzed oligosaccharide transfer of sugar oxazolines. Probing substrate structural requirement.' Chemistry. A European Journal, 12, 3355-3364 (2006).

 

15. Ni. J., Song, H., Wang, Y., Stamatos, N., Wang, L. X. 'Toward a carbohydrate-based HIV-1 vaccine: Synthesis and immunological studies of oligomannose-containing glycoconjugates'. Bioconjugate Chemistry, 17, 493-500 (2006).

 

16. Li, H, Li, B., Song, H., Breydo, L., Baskakov, I. V., Wang, L. X.  'Chemoenzymatic synthesis of HIV-1 V3 domain glycopeptides carrying two N-Glycans and effects of glycosylation on the peptide's domain'. Journal of Organic Chemistry, 70, 9990-9996 (2005).

 

17. Li, B., Zeng, Y., Hauser, S., Song, H., Wang, L. X. 'Highly efficient endoglycosidase-catalyzed synthesis of glycopeptides using oligosaccharide oxazolines as donor substrates'. Journal of the American Chemical Society, 127, 9692-9693 (2005).

 

18. Wang, J., Le, N., Heredia, A., Song, H., Redfield, R., Wang, L. X. 'Modification and structure-activity relationship of a small molecule HIV-1 inhibitor targeting the viral envelope glycoprotein gp120'. Organic & Biomolecular Chemistry, 3, 1781-1786 (2005).

 

19. Stamatos, N. M., Liang, F., Nan, X., Landry, K., Cross, A. S., Wang, L. X. & Pshezhetsky, A. V. 'Endogeneous sialidase activity of human monocytes is up-regulated during cellular differentiation into macrophages'. FEBS Journal, 272, 2545-2556 (2005).

 

20. Wang, L. X., Song, H., Liu, S., Lu, H., Jiang, S., Ni, J., Li, H. 'Chemoenzymatic synthesis of HIV-1 glycopeptides. Effects of glycosylation on the antiviral activity and alpha-helix bundle-forming ability of gp41 peptide C34'. ChemBioChem, 6, 1068-1074 (2005).

 

21. Hauser, S., Song, H., Li, H., Wang, L. X. 'A novel fluorescence-based assay for the transglycosylation activity of endo-beta-N-acetyl-glucosaminidases'. Biochemical & Biophysical Research Communications, 328, 580-585 (2005).

 

22. Li, H., Singh, S., Song, H., Zeng, Y., Wang, L. X. 'Chemoenzymatic synthesis of CD52 glycoprotein carrying native N-glycans'. Bioorganic and Medicinal Chemistry Letters, 15, 895-898 (2005).

 

23. Wang, L. X., Heredia, A., Song, H., Zhang, Z., Yu, B., Davis, C. & Redfield, R. 'Resveratrol glucuronides as the metabolites of resveratrol in humans: characterization, synthesis, and anti-HIV activities'. Journal of Pharmaceutical Sciences, 93, 2448-2457 (2004).

 

24. Li, H., Song, H., Heredia, A., Le, N., Redfield, R., Lewis, G. K., & Wang, L. X. 'Synthetic bivalent CD4-mimetic miniproteins show enhanced anti-HIV activity over the monovalent miniprotein'. Bioconjugate Chemistry, 15, 783-789 (2004).

 

25. Ni, J., Powell, R., Baskakov, I. V., DeVico, A., Lewis, G. K. & Wang, L. X. 'Synthesis, conformation, and immunogenicity of monosaccharide-centered multivalent HIV-1 gp41 peptides containing the sequence of DP178'. Bioorganic & Medicinal Chemistry, 12, 3141-3148 (2004).

 

26. Naicker, K. P., Li, H., Heredia, A., Song, H., & Wang, L. X. Design and synthesis of Gal-conjugated peptide T20 as novel antiviral agent for HIV-immunotargeting. Organic & Biomolecular Chemistry, 2, 660-664 (2004).

 

27. Wang, L. X., Ni, J., Singh, S. & Li, H. 'Binding of high-mannose type oligosaccharides and synthetic oligomannose clusters to human antibody 2G12. Implications for HIV-1 vaccine design'. Chemistry & Biology, 11, 127-134 (2004).

 

28. Li, H. & Wang, L. X. 'Design and synthesis of a cholic acid-based oligomannose cluster as the epitope mimic for human HIV-neutralizing antibody 2G12'. Organic & Biomolecular Chemistry, 2, 483-488 (2004).

 

29. Naicker, K. P., Jiang, S., Lu, H., Ni, J., Boyer-Chatenet, L., Wang, L. X. & Debnath, A. K. “Synthesis and anti-HIV-1 activity of 4-[4-(4,6-bisphenylamino-[1,3,5]triazin-2-ylamino)-5-methoxy-2-methylphenylazo]-5-hydroxynaphthalene-2,7-disulfonic acid and its derivatives”. Bioorganic & Medicinal Chemistry, 12, 1215-1220 (2004).