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CONTACT INFORMATION
Office: IHV/UMD School of Medicine, 725 Lombard St.,
Phone: 410-706-4982
Professional Interests
Publications
Honors/Awards
Funding
Courses Taught
Research Group
Lai-Xi Wang
Professor
Ph.D. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 1991; Post-Doctoral Johns Hopkins University 1994; Post-Doctoral Massachusetts Institute of Technology (MIT) 1997
SELECTED PUBLICATIONS
 
1.      Huang, W., Giddens, J., Fan, S. Q., Toonstra, C., Wang, L. X., “Chemoenzymatic glycoengineering of intact IgG antibodies for gain of functions”, J. Am. Chem. Soc., 134, 12308−12318 (2012).
 
2.      Cross, A.S., Hyun, S.W., Miranda-Ribera, A., Feng, C., Liu, A., Nguyen, C., Zhang, L., Luzina, I.G., Atamas, S.P., Twaddell, W.S., Guang, W., Lillehoj, E.P., Puche, A.C., Huang, W., Wang, L.X., Passaniti, A., Goldblum, S.E. “NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia. NEU1 restrains endothelial cell migration whereas NEU3 does not”, J. Biol. Chem., 287, 15966-15980 (2012).
 
3.      Fan, S., Huang, W., Wang, L. X. “Remarkable transglycosylation activity of glycosynthase mutants of Endo-D, an endo-β-N-acetylglucosaminidase from Streptococcus pneumonia”, J. Biol. Chem., 287, 11272–11281 (2012).
 
4.      Lillehoj, E.P., Hyun, S.W., Feng, C., Zhang, L., Liu, A., Guang, W., Nguyen, C., Luzina, I.G., Atamas, S.P., Passaniti, A., Twaddell, W.S., Puché, A.C., Wang, L.X., Cross, A.S., Goldblum, S.E. “NEU1 sialidase expressed in human airway epithelia regulates epidermal growth factor receptor (EGFR) and MUC1 protein signaling”, J. Biol. Chem., 287, 8214-8231 (2012).
 
5.      Wang, L. X., Lomino, J. V. “Emerging technologies for making glycan-defined glycoproteins”, ACS Chemical Biology, 7, 110-122 (2011).
 
6.      McLellan, J., Pancera, M., Carrico, C., Gorman, J., Julien, J. P., Khayat, R., Louder, R., Pejchal, R., Sastry, M., Dai, K., O’Dell, S., Patel, N., Shahzad-ul-Hussan, S., Yang, Y., Zhang, B., Zhou, T., Zhu, J., Boyington, J. C., Chuang, G. Y., Diwanji, D., Georgiev, I., Kwon, Y. D., Lee, D., Louder, M. K., Moquin, S., Schmidt, S. D., Yang, Z. Y., Bonsignori, M., Crump, J. A., Kapiga, S. H., Sam, N. E., Haynes, B. F., Burton, D. R., Koff, W. C., Walker, L. M., Phogat, S., Wyatt, R., Orwenyo, J., Wang, L. X., Arthos, J., Bewley, C. A., Mascola, J. R., Nabel, G. J., Schief, W. R., Ward, A. B., Wilson, I. A., Kwong, P. D., “Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9”, Nature, 480, 336-343 (2011).
 
7.      Zou, G., Ochiai, H., Huang, W., Yang, Q., Li, C., Wang, L. X., “Chemoenzymatic synthesis and Fc receptor binding of homogeneous glycoforms of antibody Fc domain. Presence of a bisecting sugar moiety enhances the affinity of Fc to FcgIIIa receptor”, J. Am. Chem. Soc., 133, 18975-18991 (2011).
 
8.      Amin, M. N., Huang, W., Mizanur, R. M., Wang, L. X., “Convergent synthesis of homogeneous Glc1Man9GlcNAc2-protein and derivatives as ligands of molecular chaperones in protein quality control”, J. Am. Chem. Soc., 133, 14404–14417 (2011).
 
9.      Huang, W., Li, J., Wang, L. X., “Unusual transglycosylation activity of Flavobacterium meningosepticum endoglycosidases enables convergent chemoenzymatic synthesis of core fucosylated complex N-glycopeptides”, ChemBioChem, 12, 932-941 (2011).
 
10. Huang, W., Zhang, X., Ju, T., Cummings, R.D., Wang, L.X. “Expeditious chemoenzymatic synthesis of CD52 glycopeptide antigens”, Org. Biomol. Chem., 8, 5224-5233 (2010).
 
11. Brooks, C.L., Schietinger, A., Borisova1, S.N., Kufer, P., Okon, M., Hirama, T., MacKenzie, C.R., Wang, L.X., Schreiber, H., Evans, S.V., “Antibody recognition of a novel tumor-specific glycopeptide antigen”, Proc. Natl. Acad. Sci. USA, 107, 10056-10061 (2010).
 
12. Huang, W., Yang, Q., Umekawa, M., Yamamoto, K., Wang, L.X., “Arthrobacter endo-beta-N-acetylglucosaminidase shows transglycosylation activity on complex type N-glycan oxazolines. One-pot conversion of ribonuclease B to sialylated ribonuclease C”. ChemBioChem, 11, 1350-1355 (2010).
 
13. Yang, Q., Li, C., Wei, Y., Huang, W., Wang, L. X., “Expression, glycoform characterization, and antibody-binding of HIV-1 V3 glycopeptide domain fused with human IgG1-Fc”, Bioconjugate Chem., 21, 875-883 (2010).
 
14. Schwarz, F., Huang, W., Li, C., Schulz, B. L., Lizak, C., Palumbo, A., Numao, S., Neri, D., Aebi, M., Wang, L. X. “A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation”, Nat. Chem. Biol., 6, 264-266 (2010).
 
15. Umekawa, M., Li,C., Higashiyama, T., Huang, W., Ashida, H., Yamamoto, K., Wang, L. X., “Efficient glycosynthase mutant derived from Mucor hiemalis endo-b-N-acetylglucosaminidase capable of transferring oligosaccharide from both sugar oxazoline and natural N-glycan”, J. Biol. Chem., 281, 511-521 (2010).
 
16. Huang, W., Wang, D., Yamada, M., Wang, L. X., “Chemoenzymatic synthesis and lectin array characterization of a class of N-glycan clusters”, J. Am. Chem. Soc., 131, 17963-17971 (2009).
 
17. Huang, W., Li, C., Li, B., Umekawa, M., Yamamoto, K., Zhang, X., Wang, L. X., “Novel glycosynthases enable a highly efficient chemo-enzymatic synthesis of N-glycoproteins carrying intact natural N-glycans”, J. Am. Chem. Soc., 131, 2214-2223 (2009).
 
18. Zhu, X. Y., Holtz, B., Wang, Y., Wang, L. X., Orndorff, P. E., Guo, A., „Quantitative glycomics from fluidic glycan microarrays“, J. Am. Chem. Soc., 131, 13646-13650 (2009).
 
19. Wang, L. X., “Expanding the repertoire of glycosynthases”, Chem. Biol., 16, 1026-1027 (2009).
 
20. Newsom-Davis, T. E., Wang, D., Steinman, L., Chen, P. F. T., Wang, L. X., Simon, A. K., Screaton, G. R., “Enhanced immune recognition of cryptic glycan markers in human tumors”, Cancer Research., 69, 2018-25 (2009).
 
21. Wang, L. X., Huang, W., “Enzymatic transglycosylation for glycoconjugate synthesis”, Curr. Opin. Chem. Biol., 13, 592–600 (2009).
 
22. Huang, W., Groothuys S., Heredia A., Kuijpers B.H., Rutjes F.P., van Delft F.L., Wang L.X., “Enzymatic glycosylation of triazole-linked GlcNAc/Glc-peptide: Synthesis, stability, and anti-HIV activity of triazole-linked HIV-1 gp41 glycopeptide C34 analogues”, ChemBioChem, 10, 1234-1242 (2009).
 
23. Huang, W., Ochiai, H., Zhang, X., Wang, L. X., “Introducing N-glycans into natural products through a chemoenzymatic approach”, Carbohydr. Res., 343, 2903-2913 (2008).
 
24. Wei, Y., Li, C., Huang, W., Li, B., Strome, S. E., Wang, L. X., “Glyco-engineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation”, Biochemistry, 47, 10294-10304 (2008).
 
25. Ochiai, H., Huang, W., Wei, Y., Wang, L. X., “Expeditious chemoenzymatic synthesis of homogeneous N-glycoproteins carrying defined oligosaccharide ligands”. J. Am. Chem. Soc., 130, 13790-13803 (2008).
 
26. Li, C., Huang, W., Wang, L. X., “Chemoenzymatic synthesis of N-linked neoglycoproteins through a chitinase-catalyzed transglycosylation”, Bioorg. Med. Chem.,16, 8366-8372 (2008).
 
27. Li, B., Takegawa, K., Suzuki, T., Yamamoto, K., Wang, L. X., “Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-N-acetylglucosaminidases”, Bioorg. Med. Chem., 16, 4670-4675 (2008).
 
28. Wang, L. X., “Chemoenzymatic synthesis of glycopeptides and glycoproteins through endoglycosidase-catalyzed transglycosylation”, Carbohydr. Res., 343, 1509-22 (2008).
 
29. Umekawa, M., Huang, W., Li, B., Fujita, K., Ashida, H., Wang, L. X., Yamamoto, K., “Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities”, J. Biol. Chem., 283, 4469-4479 (2008).